MSC Engineering Using mRNA

Mesenchymal stem cells (MSCs) are a promising cell type that can be generated naturally or through cell engineering, having a series of advantages, such as ease of isolation and expansion from many tissues, broad range of differentiation capacity, immunomodulatory and regenerative properties. To promote the widespread clinical application of mesenchymal stem cell therapies, engineering of MSCs with IVT mRNA is an attractive way in terms of overcoming translation barriers in MSC therapies and conferring cells with enhanced therapeutic efficacy. Creative Biogene has extensive experience to offer mesenchymal stem cell engineering services using IVT mRNA. We provide efficient nonviral mRNA delivery to MSCs, including electroporation, microinjection, and nanoparticle delivery system. These nonviral delivery methods are safer and more scalable and flexible than viral methods.

Mesenchymal stem cells (MSCs)

MSCs are multipotent adult stem cells that can be easily derived from different tissues, such as the umbilical cord, bone marrow, liver, adipose tissue, and induced pluripotent stem cells (iPSCs). MSCs have migration capacity and show tri-lineage multipotent in vitro (differentiation into bone, cartilage, and adipocytes). In addition, MSCs can promote angiogenesis, tissue remodeling, and modulation of the immune response via the secretion of a huge variety of proteins (such as growth factors, cytokines). In comparison to other cells, MSCs emerge as a very attractive cell type for regenerative medicine due to their availability as well as their harvesting without ethical problems. This type of cell can be easily propagated in in vitro environments. The use of autologous MSCs is a convenient solution that can obviate many of the problems in terms of adequate cell donor match and immunological aspects. Therefore, MSCs have been widely used in cell-based therapies for the treatment of many diseases.

Fig1. Mesenchymal stem cells are multipotent stromal cells.

The next-generation of MSCs engineering

Engineering of MSCs using mRNA has been proposed as a temporary and burst approach to regulating the migratory properties of MSCs through homing proteins. The method can treat MSCs with the strategies of cell surface modification with permanent changes, including DNA-based genetic modifications, enzymatic, and chemical modification. Applications of MSCs engineered using mRNA include cellular differentiation, reprogramming, promoting transplantation survival, directing tissue homing, cancer therapies, and secretion of therapeutic biomolecules.

mRNA-based MSC engineering in Creative Biogene

Creative Biogene offers a customized engineering service of MSCs using IVT mRNA. Our customized service has a strong capability to provide comprehensive technical support that includes the design of engineered MSCs, MSC engineering using mRNA transfection (with non-viral technologies), and evaluation of the engineered stem cells’ functions in vitro and in vivo. With years of experience in the mRNA research field, we are devoted to offering high-quality genetically engineered MSCs, to assist our global customers to enable the scalable translation of engineered MSC therapies for various applications, including,

• Tissue engineering, especially for bone engineering.
• Reprogramming of MSCs into iPSCs.
• Facilitate MSCs for cell therapies.
• Promote MSC differentiation to cell types, such as β-cell (for diabetes treatment) and endothelial cell (for vascular tissue engineering).
• Engineering the MSC secretome, involving immunomodulatory factors, growth factors, anti-tumorigenic factors, and exosomes.

Simply let us know about your research needs. We will propose the best strategy to best match your research objectives. For any requested information for our mRNA-based MSC engineering service, please contact us.