T4 RNA ligase 1
Cat.No. MRM-011
| Description | T4 RNA Ligase 1 is an ATP-dependent ligase, active on a broad range of substrates including single-strand RNA, DNA, oligoribonucleotides, oligodeoxynucleotides, as well as numerous nucleotide derivatives. The enzyme catalyzes the formation of a phosphodiester bond between a 5'-phosphoryl-terminated nucleic acid donor to a 3'-hydroxyl-terminated nucleic acid acceptor in a template-independent manner. |
| Concentration | 10U/μL |
| Storage Conditions | -20℃ |
| Storage Buffer | 10mM Tris-HCl pH 7.5, 25℃, 0.1mM EDTA, 500mM KCl, 1mM DTT, and 50% (v/v)Glycerol. |
| Source | Recombinant E.coli |
| Purity | ＞95% by SDS-PAGE |
| Quality Assurance | Free of endonuclease, exonuclease and RNase activities. |
For research use only. Not intended for any
clinical use.